Threonine Deaminase from Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of threonine tRNA from Bacillus subtilis.
A threonine tRNA was purified from Bacillus subtilis W168 by a combined use of column chromatographic systems. The nucleotide sequence was determined to be pG-C-C-G-G-U-G-U-A-G-C-U-C-A-A-U-D-G-G-D(U)-A-G-A-G-C-A-A-C-U-G-A-C-U-mo5U-G-U-t6A-A-psi-C-A-G-U-A-G-m7G-U-U-G-G-G-G-G-T-psi-C-A-A-G-U-C-C-U-C-U-U-G-C-C-G-G-C-A-C-C-AOH, where about 40 % of D20 remained unmodified as U20. It consists of 76 n...
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Purified threonine deaminase of Salmonella typhimurium was found to be monodisperse and to have a molecular weight of 194,000. The protein, in 6 M guanidine HCI and 0.1 M P-mercaptoethanol, dissociates into polypeptide chains of molecular weight 48,500. Tryptic peptide analysis implies that the four component chains of the native enzyme are of identical amino acid sequence. L-Isoleucine and L-v...
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Dissociation of the threonine deaminase of Ctostridium tetanomorfihum occurs upon exposure to heat, to alkaline pH, or to denaturing agents, or when preparations are diluted or allowed to age at -20°. Dissociation in the presence of urea, detergents, or an alkaline pH may be prevented by addition of adenosine diphosphate, threonine, phosphate buffer at pH 7.0, or monovalent or divalent cations....
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Threonine deaminase (Lthreonine hydrolyase (deaminating), EC 4.2.1.16) has been purified approximately 700-fold from extracts of Clostridium tetanomorphum. Both threonine and serine can serve as substrates, but threonine is deaminated 5 to 10 times more rapidly than serine. Pyridoxal phosphate, a reducing agent, and alkaline pH are required for the deamination of either amino acid. A plot of th...
متن کاملHeptaprenylpyrophosphate synthetase from Bacillus subtilis.
Heptaprenyl pyrophosphate synthetase was detected in partially purified extracts of Bacillus subtilis. The enzyme catalyzed the synthesis of all-trans C35 prenyl pyrophosphate from isopentenyl pyrophosphate and farnesyl or geranylgeranyl pyrophosphate, but it did not catalyze a reaction between isopentenyl pyrophosphate and either dimethylallyl or geranyl pyrophosphate. The enzyme reaction proc...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)77154-3